期刊论文

Effects of Zuogui Jiangtang Yishen Decoction on the expressions of renal VDR, CYP27B1 of MKR mice with diabetic nephropathy

中文

中华中医药杂志

1673-1727

2019

34

12

5665-5669

国家自然科学基金地区科学基金项目(N o.81860818),国家自然科学基金面上项目(No.81573956),国家自然科学基金重点项目(No.81630104) 贵州省科学技术基金项目(No.黔科合基础[2016]1012) 贵州省教育厅青年科技人才成长项目(No.黔教合KY字[2016]191号).

本校为其他机构

目的:探讨左归降糖益肾方对糖尿病肾病(DN)MKR鼠肾组织VDR、CYP27B1表达的影响。方法: 8周龄MKR鼠随机分为假手术组、模型组(高脂喂养联合单侧肾切除)、中药(左归降糖益肾方)组、阳性药物组(格列喹酮片与盐酸贝那普利片),每组15只;正常组选取同龄C57鼠10只。造模8周后灌胃给药,治疗8周。留取尿标本,心脏采血处死各组小鼠。电化学法检测空腹血糖,常规生化法检测尿蛋白含量;取新鲜肾组织,实时荧光定量PCR检测肾组织VDR、CYP27B1 mRNA的表达水平;Western blot法检测肾组织VDR、CYP27B1蛋白的表达水平;HE及电镜观察肾组织病理变化。结果:与模型组比较,中药组能升高DN小鼠体质量(P

Objective: To observe the effects of the Zuogui Jiangtang Yishen Decoction (ZJYD) on the expressions of renal VDR and CYP27B1 of MKR mice with diabetic nephropathy (DN). Methods: Eight weeks of MKR mice were randomly divided into sham operation group, model group (high-fat diet combined with unilateral nephrectomy), ZJYD group (Zuogui Jiangtang Yishen Decoction) and positive drug group (Gliclazin Tablets and Benazemate Hydrochloride), 15 mice each group; the normal group was selected from 10 C57 mice of the same age. Eight weeks after model establishment, the mice were gavaged for eight weeks....