期刊论文

Study on Yiqi Jiedu Formula inducing autophagy in nasopharyngeal carcinoma cells through PI3K/AKT/mTOR signaling pathway

中文

中华中医药杂志

1673-1727

2020

35

3

1484-1488

国家自然科学基金项目(No.81573721,No.81874408) 湖南省自然科学基金项目(No.2016JJ6117,No.2017JJ3246) 湖南省教育厅科研基金项目(No.16C1212) 湖南中医药大学研究生创新课题(No.2017CX33) 中医药防治眼耳鼻咽喉疾病湖南省重点实验室开放基金(No.2018YZD02) 湖南中医药大学“十三五”一级学科基础医学建设项目(No.06)。

本校为第一机构

目的:探讨益气解毒方对鼻咽癌细胞自噬的影响及其机制。方法:MT T检测益气解毒方对鼻咽癌CNE-2Z细胞增殖的影响。透射电镜和MDC染色观察益气解毒方诱导CNE-2Z细胞的自噬效应。Western Blot法检测Beclin-1、LC3、PI3K、p-AKT和p-mTOR蛋白表达量。结果:益气解毒方抑制CNE-2Z细胞增殖(P

Objective: To investigate the effects of Yiqi Jiedu Formula on autophagy of nasopharyngeal carcinoma cells and its mechanism. Methods: MTT was used to detect the effects of Yiqi Jiedu Formula on the proliferation of nasopharyngeal carcinoma CNE-2Z cells. Transmission electron microscopy and MDC staining were used to observe the autophagic effect of Yiqi Jiedu Formula on CNE-2Z cells. Western Blot was used to detect the expression levels of Beclin-1, LC3, PI3K, p-AKT and p-mTOR proteins in CNE-2Z cells treated with Yiqi Jiedu Formula. Results: M...