目的:探讨寿胎丸对复发性流产模型大鼠蜕膜细胞水通道蛋白(AQP)1、2表达的影响。方法:取复发性流产模型大鼠胚胎着床部位的蜕膜组织进行原代和传代培养,分别用寿胎丸含药血清、雌激素(E2)及孕激素(P)进行干预,另设空白对照组,4组药物对培养的蜕膜细胞作用72h后,通过q RT-PCR及Western blot方法检测蜕膜细胞中AQP1、AQP2 m RNA及蛋白的表达。结果:与空白对照组比较,寿胎丸组及P组AQP1、AQP2 m RNA及蛋白的表达均显著提高(P
摘要(英文):
Objective:To investigate the effects of Shoutai Pills on the expression of aquaporin 1(AQP1),aquaporin 2(AQP2)in decidual tissue of maternal-fetal interface in rats with recurrent abortion.Methods:The decidual tissue of the embryonic implantation site of rats with kidney deficiency and luteal insufficiency was cultured in primary and subculture.Estrogen(E_2),progesterone(P)and the serum containing Shoutai Pills were used to intervene.A blank control group was set up.qRT-PCR and Western blot were used to detect the expression of AQP1,AQP2 mRNA a...