期刊论文

Effects of Naotaifang on ferroptosis-lipid metabolism pathway protein expression after cerebral infarction

中文

中华中医药杂志

1673-1727

2020

35

11

5491-5494

No.81774033:国家自然科学基金 No.18C0379:湖南省教育厅科学研究项目 No.2018ZXYJH17:湖南中医药大学中西医结合开放基金项目 No.2020JJ4467:湖南省自然科学基金面上项目 No.19A378:湖南省教育厅科学研究重点项目

本校为第一机构

目的:研究脑缺血大鼠给予脑泰方(NTF)干预后对脂质代谢蛋白表达的影响。方法:将44只大鼠随机分为Sham组、MCAO组、MCAO+NTF组和MCAO+Fer-1组。采用大脑中动脉栓塞(MCAO)模型,术后12h采用Longa神经行为学评分标准记录大鼠活动;丙二醛(MDA)测定检测脑缺血部位脂质过氧化水平;Western blot及RT-PCR检测各组脑组织谷胱甘肽过氧化物酶4 (GPX4)、长链脂酰辅酶A合成酶4 (ACSL4)、磷脂胆碱酰基转移酶3 (LPCAT3)蛋白及基因表达;ROS检测各组脑组织活性氧(ROS)水平。结果:与MCAO组比较,MCAO+NTF组的神经行为学评分下降(P

Objective: To investigate the effects of Naotaifang (NTF) on lipid metabolism protein expression in cerebral ischemia rats. Methods: Forty-four SD rats were randomly divided into Sham operation group, Model group (MCAO group), Model+Naotaifang group (MCAO+NTF group) and Model+Ferrostatin-1 group (MCAO+ Fer-1 group). After cerebral ischemia for 12 hours, the behavioral activity of rat was recorded by Longa's neurobehavioral score standard; Detecting the lipid peroxidation level in ischemic area of rats in each group by MDA; The expressions of glutathione peroxidase 4 (GPX4), long-chain-fatty-ac...