期刊论文

Protective effect of Zuogui Jiangtang Jieyu Formula against damage of hippocampal neuron of NVU induced by mitophagy via mTOR pathway in diabetes mellitus with depression

中文

中华中医药杂志

1673-1727

2020

35

9

4631-4636

No.81573965:国家自然科学基金 No.81874464:国家自然科学基金 No.2017ZX09309026:重大新药创制"科技重大专项 No.2017JJ3241:湖南省自然科学基金 No.2018ZYX47:湖南中医药大学中医学一流学科开放基金

本校为第一机构

目的:基于mTOR介导的线粒体自噬探讨左归降糖解郁方对糖尿病并发抑郁症(DD)神经血管单元(NVU)中海马神经元损伤的保护作用。方法:原代分离、纯化和培养SD大鼠海马神经元(Ne)、星形胶质细胞(As)与脑微血管内皮细胞(Bm),并分别进行细胞鉴定。采用Transwell小室及高糖联合皮质酮干预构建DD海马NVU细胞模型,实验设正常组、模型组、GluR2阻断剂组、mTOR阻断剂、左归降糖解郁方含药血清组、GluR2阻断剂+左归降糖解郁方含药血清组和mTOR阻断剂+左归降糖解郁方含药血清组。采用CCK-8法检测海马神经元细胞存活率;Hoechst染色法检测海马神经元凋亡;免疫荧光检测自噬标志物LC3-II、SQSTM1、 Parkin及mTOR途...

Objective: To investigate protective effect of Zuogui Jiangtang Jieyu Formula against damage of hippocampal neuron of NVU induced by mitophagy via mTOR pathway in diabetes mellitus with depression. Methods: Hippocampal neurons, astrocytes and brain microvascular endothelial cells from SD rats were primitively isolated, purified and cultured, and immunocytochemistry staining was used to identify primitive cells. The DD model of NVU was established by applying intervention of high glucose and cortisone after hippocampal NVU co-culture system was builded by Transwell in vitro. The cultured cells ...