期刊论文

Mechanism of Sanxue Mingmu Tablet in preventing and treating proliferative vitreoretinopathy based on MAPK and PI3K/AKT signal pathways

中文

中华中医药杂志

1673-1727

2020

35

5

2355-2361

中医药防治五官科疾病湖南省重点实验室建设项目(No.2017TP1018) 湖南省中医药防治耳鼻喉疾病与视功能保护工程研究中心开放基金项目(No.2018YGCO4) 湖南省中医药管理局科研项目(No.201622) 湖南省研究生科研创新项目(No.CX2018B499,No.CX2018B490) 国家中医药管理局中医眼科学重点学科建设项目(No.国家中医药[2009]30号)

本校为第一机构

目的:通过建立兔外伤性增殖性玻璃体视网膜病变(PVR)模型,观察散血明目片对其视网膜增殖膜中磷脂酰肌醇-3(PI3K)、蛋白激酶B(AKT)、丝裂原活化蛋白激酶(MEK)、丝裂原活化细胞信号调节激酶(P38MAPK)蛋白的表达影响。方法:将42只新西兰大白兔随机分为空白(A)组、模型(B)组、散血明目片(C)组、PD98059干预(D)组、LY294002干预(E)组、MK-2206干预(F)组、混合抑制剂干预(G)组,每组6只。造模后30min,向D、E、F、G组玻璃体腔内分别注射0.1mL PD98059、LY294002、MK-2206及3种混合抑制剂。B、C组则予以玻璃体腔内注射0.1mL 0.9%氯化钠溶液。术后第1天,C组予以散血明目片10mg/kg溶液灌胃,除A组其余组以0....

Objective: To observe the effect of Sanxue Mingmu Tablet on the expression of PI3K, AKT, MEK and P38MAPK protein in the proliferative membrane of retina by establishing a rabbit model of traumatic proliferative vitreoretinopathy (PVR). Methods: Forty-two New Zealand white rabbits were randomly divided into blank group (A), model group (B), Sanxue Mingmu Tablet group (C), PD98059 group (D), LY294002 group (E), MK-2206 group (F) and inhibitor mixed group (G), each group 6 rabbits. After 30 minutes of modeling, 0.1 mL PD98059, LY294002, MK-2206 and three mixed inhibitors were injected into the vi...