期刊论文

Effects of different extracts of Xiongma Decoction on TRPV1 in trigeminal ganglion neurons of neonatal rat

中文

中华中医药杂志

1673-1727

2020

35

9

4380-4385

国家自然科学基金项目(No.81860844) 贵州省科学技术基金计划项目(No.黔科合基础[2016]1016,No.黔科合基础[2019]1038号) 贵州省教育厅青年科技人才成长项目(No.黔教合KY字[2016]185) 贵州省中医药、民族医药科学技术研究专项(No.QZYY-2016-001) 天然药物活性物质与功能国家重点实验室开放课题(No.GTZK201609)。

本校为其他机构

目的:探讨芎麻汤不同提取物对新生SD大鼠三叉神经节神经元瞬时受体电位香草酸亚型1 (TRPV1)的影响。方法:新生SD大鼠三叉神经节神经元分离、原代培养,应用TRPV1拮抗剂及芎麻汤不同提取物(石油醚部位、乙酸乙酯部位、正丁醇部位、萃取后水溶液部位)进行干预,采用RT-qPCR、Western Blot方法检测各组神经元TRPV1 mRNA、蛋白的表达情况。结果:①与空白对照组比较,芎麻汤石油醚提取物(1 × 10~(-6) mg/mL)及萃取后水提物(1 × 10~(-4) mg/mL)能显著降低正常三叉神经节神经元TRPV1 mRNA表达(P

Objective: To investigate the different extracts of Xiongma Decoction on TRPV1 in trigeminal ganglion neurons of neonatal rat. Methods: The trigeminal ganglion from newly born 3d SD rats were taken to obtain trigeminal ganglion neurons by using type I collagenase and trypsin digestion, and primary cultured neurons. After treatment of trigeminal ganglion neurons with TRPV1 antagonist and different extracts of Xiongma Decoction, RT-qPCR and Western Blot were used to detect the expression of TRPV1 mRNA and protein in each group. Results:①Compared...