期刊论文

Metabolomics research of anticoagulant effect of Pingle Zhenggu Huoxue Ling based on the ~1H-NMR

中文

中华中医药杂志

1673-1727

2020

35

01

427-430

国家中医药管理局“平乐郭氏正骨流派传承工作室”建设项目（No.[2012]228）； 河南省科技厅科技发展计划项目（No.112102310008）； 河南省中医药管理局“河南省中医临床学科领军人才培育计划”项目（No.HNZYLJ20131009）； 2016年度河南省科技攻关项目（No.162102310365）~~；

本校为第一机构

目的:采用~1H-NMR代谢组学技术研究平乐正骨活血灵(简称活血灵)干预创伤性深静脉血栓(DVT)大鼠后的代谢产物,探究其作用机制。方法:将90只大鼠随机分为空白对照组、模型组、阿司匹林组、活血灵组、NF-κB抑制剂(PDTC)组、PDTC+活血灵组,分别编号为A组、B组、C组、D组、E组、F组。A、B组予以蒸馏水灌胃,C组予以阿司匹林灌胃,D组予以活血灵灌胃,E组予以PDTC灌胃,F组为PDTC+活血灵灌胃。灌胃给药第7天,除A组,均予以DVT造模,造模后每日继续予以相应药物灌胃。第14天进行大鼠眼眶取血,采用~1H-NMR技术分析大鼠血液代谢产物。采用主成分分析和正交偏最小二乘法判别分析,研究D、E、F组间代谢物差异,筛选...

Objective: To study the mechanism of the metabolic product of Pingle Zhenggu Huoxue Ling intervention in DVT rats by ~1 H-NMR metabolomics. Methods: A total of 75 rats were randomly divided into blank control group, model group, Aspirin group, Huoxue Ling group, PDTC group, PDTC+Huoxue Ling group, respectively numbered as group A, group B, group C, group D, group E, group F. Group A and group B were given distilled water, group C was given Aspirin by gavage, group D was given the Huoxue Ling, group E was given PDTC, group F was given PDTC+Huoxu...