期刊论文

Study on preventive effect of Huoxueling on orthopedic postoperative high-risk blood coagulation status and its mechanism

中文

中华中医药杂志

1673-1727

2020

35

3

1520-1523

国家中医药管理局“平乐郭氏正骨流派传承工作室”建设项目(No.2012228) 河南省科技厅“国家非物质文化遗产——平乐郭氏正骨术的系统研究与开发”项目(No.112102310008) 河南省中医药管理局“河南省中医临床学科领军人才培育计划”项目(No.HNZYLJ20131009) 2016年河南省科技发展计划项目(No.162102310365)。

本校为第一机构

目的:观察活血灵方对大鼠下肢深静脉血栓(DVT)的预防作用及对核转录因子κB(NF-κB)表达水平的影响。方法:60只1月龄Wistar大鼠随机平均分成空白组,模型组,阳性药物组(予以阿司匹林),活血灵方低、中、高剂量组,采用创伤性造模方法制备大鼠下肢DVT;各组从造模前7d开始灌胃,每天1次,于造模后第7天取各组大鼠血液及发生血栓处的静脉组织;检测大鼠凝血功能变化,采用RT-PCR检测NF-κB(p65)、血管细胞黏附分子-1(VCAM-1)及组织因子(TF)mRNA表达变化。Western Blot法检测静脉组织检测NF-κB(p65)、 VCAM-1及TF的蛋白表达水平。结果:与模型组比较,活血灵方高、中剂量组及阳性药物组凝血指标均显著降低(P...

Objective: To observe the preventive effect of traditional Chinese medicine prescription Huoxueling (HXL) on deep venous thrombosis in rats and its effect on the expression of NF-κB. Methods: Sixty 1-month-old Wistar rats were randomly divided into blank group (purified water), model group (purified water), positive drug group (aspirin), low, medium and high dose HXL group. The deep vein thrombosis of the lower extremity of rats was prepared by traumatic modeling method, and the blood and venous tissue of the rats in each group were taken on t...