期刊论文

Study on the chemical constituents of hypoglycemic effective part from Clerodendron bungei Steud

中文

中华中医药杂志

1673-1727

2020

35

6

3178-3183

国家中药标准化项目(No.ZYBZH-Y-HUN-21) 湖南省自然科学基金项目(No.806132059036) 2013年中医药部门公共卫生专项“国家基本药物所需中药原料资源调查和监测项目”(No.财社[2013]135号)。

本校为第一机构

目的:对臭牡丹降糖有效部位化学成分进行研究。方法:臭牡丹乙醇提取物分别用石油醚、乙酸乙酯、正丁醇萃取得到3个提取部位;利用体外α-葡萄糖苷酶活性测定3个提取物的α-葡萄糖苷酶抑制活性来初步筛选臭牡丹降糖有效部位,然后通过高效液相-四级杆-飞行时间-质谱(HPLC-Q-TOF-MS/MS)研究降糖有效部位的化学成分,并通过查阅相关文献初步确定哪些化学成分具有潜在的α-葡萄糖苷酶抑制活性。结果:通过体外α-葡萄糖苷酶活性测定确定乙酸乙酯部位为臭牡丹降糖有效部位,并从该部位鉴定出29个化合物,包括12个苯乙醇苷,7个黄酮,5个酚酸以及5个其它化合物;通过查阅相关文献,初步确定化合物2~4、7、9~10、...

Objective:To determine the active chemical constituents of hypoglycemic effective parts from Clerodendron bungei Steud.Methods:Tested fractions were obtained by partitioning the ethanol extracts of Clerodendron bungei Steud using petroleumether,ethyl acetate and butyl alcohol.The active hyperglycemic parts were screened by a-glycosidase enzyme activity in vitro,then the chemical constituents of the effective fraction of hypoglycemic substances were studied by high performance liquid phase quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS/MS),and the related literature was used to dete...