期刊论文

Effects of estrogen inhibitor on ATF4/CHOP/Puma pathway in ovariectomized rats with cerebral ischemia and the intervention effect of Jiawei Naotai Formula

中文

中华中医药杂志

1673-1727

2020

35

7

3594-3597

No.2018JJ3390:湖南省自然科学基金 No.2019JJ50434:湖南省自然科学基金 No.17B196:湖南省教育厅科学研究项目 No.18C0379:湖南省教育厅科学研究项目 No.18A206:湖南省教育厅科学研究项目 No.kq1801045:长沙市科技计划 No.81904180:国家自然科学基金

本校为第一机构

目的:探讨雌激素抑制剂作用下加味脑泰方对去势脑缺血大鼠海马ERα蛋白及ATF4/CHOP/Puma通路相关蛋白的影响。方法:将30只雌性SD大鼠随机分为假手术组、模型组、ICI组、溶剂组、ICI+雌激素组、ICI+加味脑泰方组,每组5只。除假手术组外,其余各组大鼠均制备去势模型（行双侧去卵巢术）;行去卵巢术后第11天ICI+雌激素组、ICI+加味脑泰方组分别给予0.18mg/kg 17β-E2、24g/kg加味脑泰方灌胃,连续灌胃3d。在去卵巢术后第14天ICI组、ICI+雌激素组、ICI+加味脑泰方组大鼠制备脑缺血模型前10min侧脑室注射ICI182780,溶剂组给予等量的DMSO进行侧脑室注射。制备脑缺血模型后24h取海马,Western blotting检测...

Objective:To investigate the effects of Jiawei Naotai Formula on ERα protein and ATF4/CHOP/Puma pathway-related protein in hippocampus of ovariectomized rats with cerebral ischemia with the action of estrogen inhibitor.Methods:Thirty female SD rats were randomly divided into sham operation group,model group,ICI(ICI182780)group,solvent group,ICI+estrogen group and ICI+Jiawei Naotai Formula group,5 in each group.Except the sham operation group,other rats were prepared castration model(bilateral ovariectomy).On the 11th day after ovariectomy,ICI+...