期刊论文

Effects of combination of acupuncture and drug on the expression of GABAa receptor and GAT-1 in cerebral cortex of rats with limb spasm after stroke

中文

中华中医药杂志

1673-1727

2020

35

11

5764-5768

No.81673886:国家自然科学基金 No.2018JJ2294:湖南省自然科学基金 No.201828:湖南省中医药科研项目 No.CX2018B488:湖南省研究生科研创新项目

本校为第一机构

目的:观察针药结合对脑卒中肢体痉挛大鼠大脑皮质中γ-氨基丁酸a型受体（GABAa受体）和γ-氨基丁酸转运蛋白（GAT-1）表达的影响,研究其作用机制。方法:45只SD大鼠分为空白组、假手术组、模型组、西药组（巴氯芬溶液）和针药结合组（电针针刺阳陵泉、曲池+加味芍药甘草汤水煎剂）共5组,每组9只,运用改良的大脑中动脉闭塞（MACO）线栓法+内囊注射N-甲基-D-天门冬氨酶（NMDA）法制作脑卒中肢体痉挛状态模型,造模成功后第1天开始治疗,连续5d。以神经功能评分和肌张力评分评价模型和治疗效果,分别运用Western blot法和RT-PCR法检测大鼠大脑皮质中GABAa受体、GAT-1的含量和GABAa受体m RNA、GAT-1 mRN...

Objective: To observe the effects of combination of acupuncture and drug on the expression of γ-aminobutyric acid receptor a- (GABAa) and γ-aminobutyric acid transporter (GAT-1) in cerebral cortex of rats with limb spasm after stroke, and to study its mechanism. Methods: A total of 45 SD rats were randomly selected and divided into 5 groups: control group, sham operation group, model group, western medicine group (baclofen solution) and acupuncture combined with medicine group (acupuncture of Yanglingquan G 34, Quchi LI 11+ Jiawei Shaoyao Gan...