期刊论文

Research of Yiqi Jiedu Decoction combined with salinomycin on inhibiting proliferation of nasopharyngeal carcinoma stem cell

中文

中华中医药杂志

1673-1727

2020

35

3

1164-1169

No.81573721:国家自然科学基金 No.81874408:国家自然科学基金 No.2019JJ40216:湖南省自然科学基金 No.2017JJ3246:湖南省自然科学基金 No.16C1212:湖南省教育厅项目 No.06:湖南中医药大学十三五一流学科基础医学建设项目

本校为第一机构

目的:观察益气解毒方联合盐霉素对鼻咽癌干细胞增殖的影响并初步探讨其分子机制。方法:益气解毒方、盐霉素(SAL)单独或联合干预鼻咽癌干细胞CNE2-SC、5-8F-SC后,细胞计数法、平板克隆形成试验观察其增殖和克隆形成能力,Western Blot法检测相关蛋白的表达水平。结果:益气解毒方和SAL均显著抑制鼻咽癌干细胞增殖且呈现出浓度依赖性。各药物组均能显著抑制鼻咽癌干细胞增殖和克隆形成(P

Objective: To investigate the effects and molecular mechanism of Yiqi Jiedu Decoction (YQ) combined with salinomycin (SAL) on the proliferation of nasopharyngeal carcinoma stem cells. Methods: Nasopharyngeal carcinoma stem cells CNE2-SC and 5-8F-SC were obtained and treated with YQ, SAL alone or in combination. Cell counting methods, plate clonality assays were used to measure the impact of drugs on proliferation and colony forming ability of stem cells, and Western Blot were used to test detect the expression level of related proteins. Results: Both YQ and SAL significantly inhibited the prol...