期刊论文

Effects of Sema4D on inflammation induced by RSV and regulation of Kechuanning in asthmatic rats

中文

中华中医药杂志

1673-1727

2020

35

4

1724-1727

No.81674025:国家自然科学基金 No.81774368:国家自然科学基金

本校为第一机构

目的:探讨脑信号蛋白4D(Sema4D)对呼吸道合胞病毒(RSV)诱发哮喘大鼠炎症的影响及咳喘宁通过调节Sema4D对炎症的改善作用。方法:构建RSV复合卵清白蛋白(OVA)模型,实验大鼠随机分为正常组,模型组,咳喘宁低、中、高剂量组,Sema4D抗体组,予以相应药物干预。HE染色观察各组大鼠肺组织病理变化;免疫组化观察各组大鼠肺及脊髓组织Sema4D的表达;ELISA检测白细胞介素4(IL-4)、白细胞介素6 (IL-6)、白细胞介素8(IL-8)、肿瘤坏死因子α(TNF-α)、干扰素γ(IFN-γ)的含量。结果:与正常组比较,其余各组Sema4D表达增多,IL-4、IL-6、IL-8、TNF-α含量较高,IFN-γ含量低(P

Objective: To investigate the effects of Sema4D on RSV-induced inflammation in asthmatic rats and the regulatory effect of Kechuanning on inflammation. Methods: RSV compound OVA model was constructed, and the experimental rats were randomly divided into the normal group, the model group, the low, medium and high dose group, the Sema4D antibody group, and corresponding drug intervention was given. Pathological changes of lung tissues in each group were observed by HE staining. The expression of Sema4D in lung and spinal cord tissues was observed...