期刊论文

Effects of Zuogui Jiangtang Qingzhi Recipe on expressions of PI3K signal pathway of liver tissue in MKR mice with type 2 diabetes mellitus complicated with non-alcoholic fatty liver disease

中文

中华中医药杂志

1673-1727

2021

36

01

106-111

贵州省科学技术基金项目（No.黔科合基础[2016]1012）； 贵州省教育厅青年科技人才成长项目（No.黔教合KY字[2016]191号）； 国家自然科学基金地区科学基金项目（No.81860818）;国家自然科学基金面上项目（No.81573956）;国家自然科学基金重点项目（No.81630104）； 贵州中医药大学糖脂代谢病研究中心～～；

本校为其他机构

目的:探讨以滋阴益气、活血解毒立法的左归降糖清脂方对2型糖尿病合并非酒精性脂肪性肝病MKR鼠肝组织PI3K、AKT_(1)、mTOR表达的影响。方法:8周龄MKR鼠按性别、空腹血糖、体质量随机分为空白组、模型组、中药组、阳性药物组;同龄C57/BL/6小鼠作为正常组;每组16只;除正常组、空白组外,其余各组小鼠高脂饲养8周。各给药组予以相应药物灌胃治疗,其中中药组予左归降糖清脂方29.6g/kg灌胃治疗8周后,以心脏采血处死各组小鼠,采用电化学法检测小鼠空腹血糖,常规生化法检测肝功能、血脂;电镜及HE染色观察肝组织形态结构及病理变化;实时荧光定量PCR或Western Blot分别检测肝组织PI3K、AKT_(1)、mTOR的mRN...

Objective: To investigate the effect of Zuogui Jiangtang Qingzhi Decoction on expression levels of PI3K,AKT_1 and mTOR in liver tissue of MKR mice with type 2 diabetes mellitus complicated with nonalcoholic fatty liver disease(NAFLD).Methods: MKR mice aged 8 weeks were randomly divided into blank group,model group,traditional Chinese medicine group and positive drug group according to gender,fasting blood glucose and body weight.C57/BL/6 mice of the same age were served as normal group with 16 mice in each group.In addition to normal and blank groups,the rest of the mice were fed with high fat...