期刊论文

Effects of Fangzhan Decoction on TGF- β1/Smad signaling pathway in rats with intrauterine adhesions

中文

中华中医药杂志

1673-1727

2021

36

12

7350-7355

湖南省教育厅一般科研项目（No.18C0376,No.19C1398）； 湖南中医药大学2018年度中西医结合一流学科开放基金项目（No.2018ZXYJH09）； 湖南省研究生科研创新项目（No.CX20200796）； 湖南中医药大学研究生创新课题（No.2019CX20）~~；

本校为第一机构

目的:探讨防粘汤对宫腔粘连大鼠的作用及其可能机制。方法:将76只SD大鼠随机分为正常组(12只)、假手术组(12只)及造模组(52只),采用机械刮宫及感染双重损伤建立宫腔粘连大鼠模型,造模成功后,将造模组随机分为模型组、雌激素组(0.63mg·kg~(-1) · d~(-1))及防粘汤低、中、高剂量(4.14、8.28、16.56g·kg~(-1) ·d~(-1))组,各10只。连续灌胃给药21d后处死大鼠,收集其双侧子宫组织,肉眼观察其大体形态学改变,HE及Masson染色检测其组织病理学变化;RT-PCR及Western Blot法检测子宫组织中TGF-β1和Smad2、Smad3、Smad7 mRNA及蛋白表达。结果:与正常组比较,模型组大鼠子宫组织肿胀,弹性差,子宫指数增高...

Objective:To explore the treatment and pathogenesis of Fangzhan Decoction (FZD) in rats with intrauterine adhesion.Methods:Seventy-six SD rats were randomly divided into normal group (n=12),sham operation group (n=12) and model group (n=52).The rat models of intrauterine adhesions were established using mechanical injury and infection.After the establishment of the model,the modeling group were randomly divided into positive control group and FZD at a dose of 4.14,8.28,16.56g · kg~(-1) · d~(-1) respectively daily for 21d.The rats were then sa...