期刊论文

Mechanism research of Rehmanniae Radix Praeparata-Radix Polygonimultiflori on treating Alzheimer's disease based on network pharmacology

中文

中华中医药杂志

1673-1727

2021

36

06

3267-3272

No.81373702:国家自然科学基金 No.81874462:国家自然科学基金

本校为第一机构

目的:利用网络药理学方法探讨熟地黄-何首乌药对治疗阿尔茨海默病(AD)的作用机制.方法:经数据库查找及文献检索,获得熟地黄和何首乌的有效成分,利用STITCH、Drug Bank与TCMSP数据库得到有效成分作用靶点,并构建药物-靶标网络、PPI网络,对靶点利用Metascape数据库进行GO分析和KEGG分析.采用MTT实验、ELISA法、比色法和荧光定量PCR实验对网络药理学主要分析结果进行验证.结果:研究共筛选出熟地黄-何首乌药对的有效成分28个,对应靶点236个,与AD相关靶点共110个,KEGG相关信号通路前10条,GO分析前20个生物学过程.与对照组比较,模型组的细胞存活率降低(P＜0.01),IL-1β、IL-6和TNF-α含量均升高(P＜0....

Objective:To explore the mechanism of Rehmanniae Radix Praeparata-Radix Polygoniraultiflori in the treatment of Alzheimer's disease (AD) by using network pharmacology.Methods:Through database search and literature retrieval,the active ingredients of Radix Praeparata and Radix Polygonimultiflori were obtained,and their targets were obtained through STITCH,Drug Bank and TCMSP databases.The drugs-targets network and PPI network were built.The target protein was analyzed by GO and KEGG using Metascape database.The main results of network pharmacolo...