期刊论文

Influence of plasma containing invigorating blood and dissipating masses Chinese medicine compound on proliferation of rabbit retinal pigment epithelium cell by bFGF intervention

中文

中华中医药杂志

1673-1727

2021

36

05

2526-2529

No.81774372:国家自然科学基金 No.201622:湖南省中医药局科研项目 No.CX2016B377:湖南省研究生科研创新项目 No.CX2017B432:湖南省研究生科研创新项目 No.2017TP1018:湖南省重点实验室建设项目 No.kel704005:长沙市科技计划 :中央财政支持地方高校建设项目 :国家中医药管理局中医药重点学科建设 :湖南省中医五官科学重点学科建设项目

本校为第一机构

目的:探索活血散结中药复方含药血浆对碱性成纤维细胞生长因子（bFGF）干预下兔视网膜色素上皮（RPE）细胞增殖的影响。方法:选用对数生长期兔RPE细胞,分成空白对照组、正常血浆组、bFGF组、bFGF+BGJ398组、bFGF+含药血浆组5组,CCK法测定各组兔RPE细胞的细胞活力,流式法检测各组兔RPE细胞的凋亡率。结果:与bFGF组比较,bFGF+BGJ398组、bFGF+含药血浆组细胞活力显著抑制（P

Objective:To explore the inhibition effect of invigorating blood and dissipating masses Chinese medicine compound drug-cotaining plasma on rabbit retinal pigment epithelium(RPE)cell proliferation under intervention of basic fibroblast growth factor(bFGF).Methods:Select the rabbit RPE cells in log phase.The experiment objects were divided into the blank control group,the normal plasma group,the bFGF group,the bFGF+BGJ398 group,the bFGF+drug-containing plasma group.CCK method was used to detect the absorbance value(OD)of rabbit RPE cells from different groups.The flow method was adopted to measu...