期刊论文

中文

中华中医药杂志

1673-1727

2022

37

6

3510-3514

湖南省自然科学基金项目(No.2020JJ5428) 湖南省中医药管理局课题(No.201935) 中药学一流学科,湖南省普通高等学校中药现代化研究重点实验室。

本校为第一机构

目的:探讨异类叶升麻苷与桔梗皂苷D单用及配伍对A549细胞增殖、迁移和侵袭的影响及作用机制。方法:采用CCK-8、细胞划痕、Transwell试验分别检测细胞增殖、迁移和侵袭,Western Blot检测EphA2/AKT/mTOR通路蛋白,ELISA检测肿瘤坏死因子-α (TNF-α)、白介索-6 (IL-6)含量。结果:异类叶升麻苷和桔梗皂苷D均能抑制细胞增殖,且IC_(50)分别为141、170µg/mL。与对照组比较,桔梗组、配伍组愈合率显著下降(P

Objective:To explore the effects and mechanism of the two components of isoverbascoside and platycodin D(PD)on the proliferation,migration and invasion of A549 cells.Methods:Proliferation,migration and invasion of cells were detected by CCK-8,cell scratch and Transwell assay,EphA2/AKT/mTOR pathway protein expression was detected by Western Blot,and TNF-αand IL-6 content was detected by ELISA.Results:Isoverbascoside and PD could inhibit cell proliferation with respectively IC50 of 141 and 170μg/mL.Compared with the control group,the healing ra...