期刊论文

中文

中华中医药杂志

1673-1727

2022

37

08

4670-4674

湖南省教育厅科学研究项目(创新平台开放基金)(No.19K070) 2020年度农产品加工研究所重点实验室开放课题(No.17) 2020年度湖南省大学生创新创业训练计划项目(No.2612)。

本校为第一机构

目的:筛选新五汁饮对虚热证小鼠药效最优的组方。方法:70只雄性昆明小鼠适应性喂养7 d后随机分为正常组,模型组,六味地黄丸组,新五汁饮I、Ⅱ、Ⅲ、Ⅳ组,每组10只。甲状腺片灌胃造虚热证模型。连续2周灌胃给药。造模及给药期间检测小鼠耳温、体质量变化。末次给药后检测小鼠血常规,摘取小鼠甲状腺、肺组织,HE染色后观察组织变化情况,并进行免疫组化检测甲状腺组织、肺组织NF-κB p65 、 p-NF-κB p65、I-κB β蛋白表达变化,比较不同组方的新五汁饮对虚热证药效的影响。结果:第14天,与正常组比较,模型组小鼠体质量显著降低(P

Objective:To screen the optimal pharmacological efficacy of New Five-juice Decoction on mice with deficiency heat evidence model.Methods:Seventy male Kunming mice were randomly divided into normal group,model group,Liuwei Dihuang Pills group,New Five-juice Decoction Ⅰ,Ⅱ,Ⅲ and Ⅳ groups after 7 d of adaptive feeding,and 10 mice in each group.Gastric gavage was administered continuously for 2 weeks.The changes of ear temperature and body mass of mice were detected during the modeling and drug administration.After the last administration,the bl...