期刊论文

中文

中华中医药杂志

1673-1727

2022

37

5

2712-2717

No.30772824:国家自然科学基金 No.81574031:国家自然科学基金 No.2017TP1018:中医药防治五官科疾病湖南省重点实验室建设项目 No.ZK1801YK015:国家中医药管理局中医药重点学科建设 No.CX2018B497:湖南省研究生科研创新项目 No.CX20200783:湖南省研究生科研创新项目 :湖南中医药大学中医学国内一流建设学科建设项目

本校为第一机构

目的:研究肝经郁热型干眼患者与健康志愿者泪液之间的蛋白质组学差异。方法:纳入肝经郁热型干眼患者与健康志愿者各5例,采用数据非依赖性采集高分辨率色谱-质谱技术(DIA LC-MS)对肝经郁热型干眼患者与健康人泪液进行检测,筛选出差异蛋白,并对差异蛋白进行GO富集、KEGG通路、蛋白互作用网络等生物信息学分析。结果:干眼组与健康组比较共127个差异蛋白,其中115个上调,12个下调。肝经郁热型干眼患者差异蛋白主要涉及补体激活、经典途径,吞噬作用中的Fc γ受体信号通路等生物过程;涉及细胞外泌体、细胞外区域等细胞组分;涉及抗原结合、免疫球蛋白受体结合等分子功能。差异蛋白主要涉及PI3K-Akt信号通...

Objective:To study the proteomic differences between tears of dry eye patients with liver meridian stagnation heat and healthy volunteers.Methods:Five dry eye patients with liver meridian stagnation heat and five healthy volunteers were enrolled.The tears were detected by data independent acquisition liquid chromatography-mass spectrometry(DIA LC-MS),and the differential proteins were screened out.The bioinformatics analysis of GO enrichment,KEGG pathway and protein interaction network were carried out.Results:Compared with the healthy group,th...