期刊论文

HPLC-DAD Simultaneous Determination of Four Components in Naojian Capsules

中文

中医学报

1674-8999

2014

6

772-775

10.16368/j.issn.1674-8999.2014.06.042

国家重点基础研究发展计划（编号:2012CB723503）；

本校为第一机构

目的：建立脑健胶囊 HPLC-DAD 指纹图谱，并对其指标成分川芎嗪、阿魏酸、洋川芎内酯 A、藁本内酯进行含量测定。方法：采用 Elipse XDB-C18（250 mm ×4.6 mm，5μm）色谱柱，柱温30℃，以0.3%乙酸水和乙腈为流动相进行梯度洗脱，流速1.0 mL·min -1，采用 DAD 检测器，检测波长为川芎嗪295 nm，阿魏酸325 nm，洋川芎内酯 A 280 nm，藁本内酯328 nm。结果：脑健胶囊中4种指标成分能实现同时分离，川芎嗪、阿魏酸、洋川芎内酯 A、藁本内酯的线性范围分别为：0.1033~2.066 g （R2＝0.9999），0.04966~1.9864 g（R2＝0.9995），0.1011~2.022 g（R2＝1），0.4072~2.066 g（R2＝1）；平均加样回收率...

Objective ：To establish an HPLC-DAD method for acquiring fingerprint of Naojian capsules, also simuhaneous determination of ligustrazine,ferulaic acid, senkyunilide A and ligustilide. Methods：The analysis was carried out on Elipse XDB-C18 （250 mm× 4.6 mm,5 μm） column with acetonitrile- 0.3% acetic acid solution as mobile phase for gradient elution, the column temperature was maintained at 30℃ ;The flow rate was 1.0 ml/min;The detection wavelength was set at 295 nm,325 nm ,280 nm,328 nm separately. Results ：The four compounds in their re...