期刊论文

Effects of Two Different Dosage Forms of Sijunzi Cream on Gastrointestinal Function,AchE and NOS lLevels in Spleen Qi Deficiency Model Rats

中文

中医学报

1674-8999

2020

35

2

348-352

10.16368/j.issn.1674-8999.2020.02.079

华润三九集团横向合作项目(5001/0153) 湖南中医药大学研究生创新课题项目(2018CX27)。

本校为第一机构

目的:观察颗粒剂四君子膏和传统四君子膏对脾气虚模型大鼠胃肠功能、Ach E及NOS水平的影响。方法:60只SD大鼠(雌雄各半)随机分为正常组对照、模型对照组、颗粒剂四君子膏组(颗粒膏组)、传统四君子膏方组(传统膏组)、四君子汤组(汤剂组)。采用耗气破气+劳倦过度+饥饱失常法连续造模3周,复制脾气虚证大鼠模型,颗粒膏组、传统膏组、四君子汤组分别给予相应药物10 mL·kg^-1灌胃4周。第4周末,各组大鼠腹主动脉取血,取空肠组织,HE染色观察大鼠空肠组织病理形态学变化;通过酶联免疫法、免疫组织化学法检测各组大鼠乙酰胆碱酯酶(acetylcholin esterase,AchE)、一氧化氮合酶(nitric oxide synthase,NOS)...

Objective:To observe the effects of granules Sijunzi Cream and traditional Sijunzi paste on gastrointestinal function,AchE and NOS levels in spleen qi deficiency rats.Methods:Sixty SD rats(male and female)were randomly divided into normal group control,model control group,granule Sijunzi cream group(granular cream group),traditional Sijunzi Cream prescription group(traditional cream group),and Sijunzi decoction group(decoction).The method of Qi-brokend+over-exhaustion+hungersatisfaction was used to establish the model for 3 weeks,and the rat model of Spleen Qi deficiency syndrome was establish...