期刊论文

Effect of Yiqi Jiedu decoction on apoptosis and proliferating in developing process of carcinogenesis in rats

中文

湖南中医药大学学报

1674-070X

2009

29

2

10-13

10.3969/j.issn.1674-070X.2009.02.004

国家自然科学基金资助项目（30572408）； 湖南省自然科学基金资助项目（07JJ6041）；

本校为第一机构

目的 探讨益气解毒方对大鼠鼻咽癌变过程中细胞凋亡和增殖的干预作用.方法 用二亚硝基哌嗪(DNP)诱导大鼠诱发鼻咽癌变模型,益气解毒方进行干预治疗.定期分批处死大鼠,取鼻咽组织做病理检测,TUNEL法检潮细胞凋亡活性,免疫组织化学SABC法检测PCNA蛋白活性表达.结果 益气解毒方组大鼠鼻咽上皮组织的癌变率为0%.明显低于生理盐水模型组(86.3%);维甲酸阳性对照组大鼠的癌变率为0%,但该组所有动物均于实验周期的第250～310天间逐渐死亡.在诱发性鼻咽上皮细胞癌变进程中的单纯性增生阶段,大鼠鼻咽上皮细胞凋亡活性和PCNA蛋白表达活性组间差异均无统计学意义(P＞0.05);在异型增生阶段,益气解毒方组鼻咽上...

Objective To investigate the effects of Yiqi Jiedu decoction（YJD） on the apoptosis and proliferating in the developing process of carcinogenesis in rats. Mehtods The nasopharyngeal carcinoma （NPC） models were induced by N. N＇-dinitrosopiperazine （DNP）, treated with YJD, and killed regularly to get the nasopharyngeal tissue for pathohistological evaluation. Apoptotic activities was detected with TUNEL（TdT-mediated x-dUTP nick end labeling） and the activity expression of PCNA with SABC immunohistochemical. Results The rate of cancerously...