会议论文

Primary Exploration of Metabolic Characteristics in Poria cocos fermentation system with adding botanical drug factors

中文

2012

140-146

2012全国中医药生物工程技术研究与应用交流研讨会

2012全国中医药生物工程技术研究与应用交流研讨会论文集

2012-09-14

拉萨

中华中医药学会

本校为第一机构

目的:对植物药-茯苓菌共发酵体系进行初步研究.方法:通过菌株筛选、碳源初筛、氮源初筛等确定茯苓菌发酵培养基,并将12种常见植物药粉末添加到茯苓菌发酵体系观察期其对茯苓胞外多糖合成和菌丝体生长的影响.结果:茯苓菌ZK表现出较强的胞外多糖合成能力和细胞生长能力,2％葡萄糖+1％玉米粉为最佳碳源,0.5％酵母膏为最佳氮源;黄芪、茶叶、麦芽、三七、葛根、穿心莲表现出极强的胞外多糖合成促进能力,黄芪、茶叶、穿心莲、鹿衔草可显著促进茯苓菌丝体的生长,但鹿衔草却抑制了茯苓胞外多糖的合成或分泌;而枸杞、山楂、丹参、茜草、连翘则抑制茯苓的生长.结论:植物药因子对茯苓菌的胞外多糖合成和菌丝...

　　Objective: To study the metabolic characteristics of Poria cocos fermentation system with adding botanical drug factors.Methods: Using the biomass yield and exopolysaccharide concentration as the main evaluation to choose the excellent Poria cocos strain and to determine the best carbon source and the best nitrogen source for Poria cocos fermentation.Then twelve botanical drug factors were added to Poria cocos fermentation system for studying the metabolic characteristics.Results: Poria cocos ZK was chosen as the beststrain for Poria cocos ...