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Molecular cloning and spatiotemporal expression of APETALA1-like gene in Lonicera macranthoides

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成果类型:
期刊论文
作者:
Wang, Shan;Peng, Mei Chen;Chen, Xun;Liu, Chang Yu;Chen, Ya;...
通讯作者:
Liu, Xiang Dan;Zhou, Ri Bao
作者机构:
[Liu, Xiang Dan; Zhou, RB; Liu, Chang Yu; Zhou, Ri Bao; Chen, Xun; Chen, Ya; Peng, Mei Chen; Wang, Shan] Hunan Univ Chinese Med, Coll Pharm, Changsha, Hunan, Peoples R China.
[Wang, Shan] Hunan Univ Chinese Med, Affiliated Hosp 2, Pharm, Changsha, Hunan, Peoples R China.
通讯机构:
[Liu, XD; Zhou, RB] H
Hunan Univ Chinese Med, Coll Pharm, Changsha, Hunan, Peoples R China.
语种:
英文
关键词:
APETALA1 gene;bioinformatics;fluorescence quantification;cloning;Lonicera macranthoides Hand;-Mazz
期刊:
Journal of Genetics
ISSN:
0022-1333
年:
2018
卷:
97
期:
5
页码:
1281-1288
基金类别:
Chinese National Natural Science FoundationNational Natural Science Foundation of China (NSFC) [81203007, 81673546]; Natural Science Foundation of China Hunan Province [2017JJ3237]; Youth Foundation of China Hunan Provincial Education Department [16B193]; Key Disciplines Foundation Program of Chinese Medicine in China Hunan Province [zy201505]; 'Hunan Province Universities 2011 Collaborative Innovation Center of Protection and Utilization of Hu-xiang Chinese Medicine Resources' (Xiang teach [2015]) [351]
机构署名:
本校为第一且通讯机构
院系归属:
第二中医临床医学院
药学院
摘要:
APETALA1 (AP1), a floral meristem identity gene controls the flowering time and floral transition, and plays an important role in inflorescence and floral organ development. The full-length cDNA for AP1 was obtained by rapid amplification of the cDNA ends (RACE) so that the roles of AP1 in Lonicera macranthoides (Lm-AP1) could be better understood. AP1 (accession number in GenBank: MF418642) consisted of a 729-bp open reading frame encoding a protein that contained 242 amino acids, had a deduced molecular mass of 27.9919 kDa and a theoretical i...

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