Objective: Tu Jia ethnomedicine is a unique medical system inherited and adhibited by Tu Jia minority living in central-south China. Panax japonicus C. A. Mey. (Bai San Qi, 白三七) is recognized as an effective and rare medicinal plant to treat weakness, fatigue and rheumatism in Tu Jia ethnomedicine. This paper is to discover more substance evidence for the application of Tu Jia ethnomedicine. Methods: Column chromatography and preparative high performance liquid chromatography (HPLC) was applied for isolation and purification; 1H-NMR, 13C-NMR...