期刊论文

Li, Z.

中文

中国粮油学报

1003-0174

2014

29

1

52-56+60

国家林业局948计划（2011-4-17）； “十一五”国家科技支撑（2009BADB1B10）；

本校为其他机构

以油茶粕为试验原料,通过摇瓶发酵优化产枯草芽孢杆菌的工艺条件。首先通过单因素试验探讨接种量、初始pH、摇瓶装液量、摇床转速、发酵温度和时间对产枯草芽孢杆菌活菌数的影响,在此基础上选择发酵温度、摇床转速、发酵时间3个主要影响因素进行Box-Behnken设计,得到了枯草芽孢杆菌发酵最优工艺条件。结果显示:500 mL摇瓶装培养基120 mL、发酵初始pH 6.8、接种9 mL菌悬液、摇床转速180r/min、发酵温度37℃、发酵时间37 h,发酵液中活菌数可达2.04×1010CFU/mL。

By camellia meal as experimental material, the fermentation conditions for Bacillus subtilis production have been optimized on shaking-flask level. Firstly, monofactorial experiments were applied to analyze the effects of inoculation amount, initial pH, amount of medium per flask, table concentrator rotation speed, fermentation temperature and fermentation period on Bacillus subtilis production. Based on the results of monofactorial experiments, fermentation temperature, table concentrator rotation speed and fermentation period were selected to...