期刊论文

JiangzhiLigan Decoction reduce activation of NLRP3 inflammasome and attenuate high-fat diet-induced nonalcoholic fatty liver disease in rats

中文

中国免疫学杂志

1000-484X

2020

36

22

2716-2720

10.3969/j.issn.1000-484X.2020.22.006

81503385:国家自然科学基金 C2017028:湖南省卫计委科研计划项目 18B244:湖南省教育厅科学研究项目 18B252:湖南省教育厅科学研究项目

本校为第一机构

目的:探讨降脂理肝汤对非酒精性脂肪肝病(NAFLD)大鼠NLRP3炎症小体活化的影响及机制。方法:利用高脂饮食喂养大鼠构建NAFLD模型,分别灌胃给予2.3、4.6、9.2 g/kg降脂理肝汤和10 mg/kg NLRP3特异性抑制剂MCC950,连续6周,全自动生化分析仪检测大鼠血清ALT和AST水平,HE染色观察大鼠肝脏组织病理学变化,Western blot检测大鼠肝脏组织NLRP3、Caspase-1、IL-1β、和IL-18蛋白表达水平。结果:与正常组相比,模型组大鼠血清ALT和AST水平显著升高,肝细胞脂肪变性和脂质沉积明显,气球样变评分和NAFLD活动度评分显著升高,肝脏组织NLRP3、Caspase-1、IL-1β、和IL-18蛋白表达水平显著升高;降脂理肝汤和MCC950...

Objective:To explore effect and mechanism of JiangzhiLigan Decoction on NLRP3 inflammasome activation of rats with non-alcoholic fatty liver disease (NAFLD).Methods:NAFLD rat model was established by high-fat diet feeding,orally administered with 2.3,4.6,9.2 g/kg of JiangzhiLigan Decoction and 10 mg/kg of NLRP3 specific inhibitor MCC950 for 6 weeks,respectively.Serum levels of ALT and AST were determined by automatic biochemical analyzer.Liver tissues and histopathological changes were stained by HE.Western blot was used to detect expression le...