期刊论文

Determination of Berberin in Kangpenyan Capsule

中文

中国实验方剂学杂志

1005-9903

2008

14

1

6-7

10.3969/j.issn.1005-9903.2008.01.004

本校为第一机构

目的:建立反相高效液相法测定康盆炎胶囊中小檗碱含量的方法.方法:采用十八烷基硅烷键合硅胶柱,流动相为甲醇-乙腈-水(40:21:39)(内含0.8 g十二烷基磺酸钠和0.5 g酒石酸);检测波长为346 nm.结果:小檗碱在(0.083～0.747)μg范围内呈良好的线性关系;平均回收率为96.34%,RSD为0.98%.结论:该方法不仅操作简单、准确,且重复性好,可用于康盆炎胶囊的质量控制.

Objective： To develop a RP-HPLC method for determination of berberin in Kangpenyan Jiaonang. Methods： Berberin was separated completely on an C18 column, using a mixture of methanol, acetonitrile and water（40：21：39） （containing 0.8 g Sodium dodecyl sulphonate and 0.5 g tartaric acid） as mobile phase, with UV detection at 346 nm. Results：The linear range of berberin was in （0.083-0.747）μg, the recovery rate was 96.34%, RSD=0.98% . Conclusions：The method is very stable, accurate, and repeatable. It can be ...