期刊论文

Qualitative and Quantitative Analysis of Salviaflaside in Prunella vulgaris by TLC and HPLC

中文

中国实验方剂学杂志

1005-9903

2012

18

21

106-108

10.13422/j.cnki.syfjx.2012.21.066

湖南省科技厅项目（2011FJT008） 湖南省教育厅项目（11C0958）

本校为第一机构

目的：建立夏枯草中异迷迭香酸苷的TLC鉴别和HPLC含量测定方法,为完善夏枯草质量标准提供参考。方法：采用薄层色谱法对夏枯草及夏枯草不同部位中的异迷迭香酸苷进行定性鉴别;采用高效液相色谱法对夏枯草中异迷迭香酸苷进行含量测定,以Agilent Eclipse XDB-C18（4.6 mm×250 mm,5μm）为色谱柱,流速1.0 mL.min-1,检测波长319 nm,柱温30℃,进样体积10μL,流动相16%乙腈-84%水（含1.0%醋酸）等度洗脱。结果：异迷迭香酸苷斑点清晰,且仅存在药用部位果穗中,无干扰;异迷迭香酸苷进样在0.032 667～0.653 34μg与峰面积线性关系良好（r=0.999 9）,平均回收率为98.18%,RSD 0.25%,异迷迭香酸苷在夏枯草中...

Objective： To establish an effective TLC and HPLC method for qualitative and quantitative analysis of salviaflaside in P. vulgaris which can be used to be one of the quality control of P. vulgaris. Method： Salviaflaside was identified by TLC in P. vulgaris and different parts from P. vulgaris; salviaflaside was determined by RP-HPLC. Chromatography conditions were Agilent Eclipse XDB-C18 （4.6 mm × 250 mm, 51μm） column with mobile phase of acetonitrile-1.0% acetic acid （16： 84）, UV detection wavelength was set at 319 nm and the column t...