期刊论文

中文

中国实验方剂学杂志

1005-9903

2012

18

4

103-106

10.3969/j.issn.1005-9903.2012.04.027

本校为第一机构

目的:研究西藏红雪茶多糖含量测定的方法.方法:采用苯酚-硫酸法测定总糖含量,3,5-二硝基水杨酸(DNS)法测定还原糖含量,两者的差值即为多糖含量.结果:总糖标准曲线方程为OD =0.051 4C- 0.0146(r=0.999 2),还原糖标准曲线方程为OD =0.022 9C-0.086(r =0.999 9),两者分别在2.5 ～12.5,8～40 mg·L-1有良好的线性关系.测得LC,LCA,LCP多糖含量分别为63.6％,82.6％,57.6％.结论:该方法操作快速、简便、灵敏、准确、重复性好.

Objective：To establish a method for the determination of polysaccharide in Lethariella cladonioides.Method：Using microplate reader,the content of total sugar was determined by means of phenol-sulfuric acid method and 3,5-dinitro salicylic acid（DNS） method was used for the determination of reducing sugar,the difference between the two was the polysaccharide content.Results：The total sugar standard curve equation was OD=0.051 4 C-0.014 6（r=0.999 2） and reducing sugar standard curve equation was OD =0.022 9C-0.086（r=0.999 9）.Respectively ...