期刊论文

Determination of Paeonol Concentration in Eczema Nanoemulsion Spray by HPLC

中文

中国实验方剂学杂志

1005-9903

2012

18

24

97-100

10.13422/j.cnki.syfjx.2012.24.028

国家科技部“十一五”支撑计划(2008BAI53B042)；

本校为第一机构

目的:建立湿疹纳米乳喷雾剂中丹皮酚的HPLC定量方法,控制该制剂的质量。方法:采用高效液相色谱法,Phenomenex C18-ODS色谱柱,流动相甲醇-水(55∶45),流速1.0 mL.min-1,检测波长274 nm。结果:标准曲线线性范围在10.0～100.0 mg.L-1(r=0.999 9),48 h内稳定,加样回收率99.60%,RSD 1.123%。理论塔板数均在6 000以上。分离度达1.5以上。结论:方法简便、快速、灵敏、准确,稳定性及重复性好,可作为该制剂中丹皮酚含量测定的常规方法。

Objective： To establish a method for the determination of paeonol in Eczema Nanoemulsion spray by HPLC. Method： Using HPLC technique, we detected the samples by the C18 ODS column of Phenomenex company under the wave length at 274 nm, the chromatographic conditions were phenomenex C18-ODS column, mobile phase of methanol-water （55：45） , flow rate of 1.0 mL ·min^-1 and detection wave-length at 274 nm. Result： The standard curve was linear over the range of 10.0-100.0 mg ·L^-1, with the correlation coefficient of 0. 999 9. It is steady in...