期刊论文

Isolation,Purification and Monosaccharide Analysis of an Acidic Polysaccharide from Polygonatum odoratum

中文

中国实验方剂学杂志

1005-9903

2013

19

9

69-72

10.11653/syfj2013090069

国家科技部支撑计划项目（2011BA101B09）；

本校为第一机构

目的:对玉竹粗多糖进行分离纯化,并对所得均一多糖进行定性分析.方法:采用热水提取玉竹粗多糖,乙醇分级沉淀,收集乙醇体积分数为80％条件下析出的多糖沉淀物.再经DEAE-52纤维素离子交换层析和Sephadex G-100分子筛色谱得到玉竹酸性多糖(POAPS0).应用紫外光谱、凝胶柱色谱、纸色谱分析POAP80的纯度,红外光谱和PMP柱前衍生化高效液相色谱分析其结构和单糖组成.结果:从玉竹粗多糖中分离纯化了1种酸性多糖POAP80,主要含有甘露糖、葡萄糖、半乳糖醛酸、半乳糖4种单糖,其摩尔比为0.93∶2.65∶ 29.38∶3.47.结论:POAP80为玉竹中新发现的均一组分多糖,上述方法提供了玉竹POAP80的一些结构信息,为进一步研...

Objective：To isolate and purify the crude polysaccharide from Polygonatum odoratum and then to identify the chemical structure.Method： A polysaccharide-rich extract was obtained by hot water extraction from Polygonatum odoratum and purified by grading ethanol precipitation and collect the precipitate at 80% volume fraction of ethanol,were sequentially purified by DEAE-cellulose chromatography and Sephadex G-100 molecular sieve chromatography and an acidic polysaccharide fraction named POAP80 was obtained.The purity of POAP80 was identified by...