期刊论文

中文

中国实验方剂学杂志

1005-9903

2014

20

1

77-80

10.11653/syfj2014010077

湖南省教育厅重点课题(07A049)； 湖南省科技厅科学研究项目(2008sk3068)；

本校为第一机构

目的:对舒胸片进行血清药物化学研究。方法:采用SD大鼠灌胃给药,于给药一定时间后取血,测定复方及各单味药提取液血样的HPLC色谱图。采用Kromasl C18色谱柱,0.05%酸水-乙腈梯度洗脱,波长203 nm,流速1 mL·mL。结果:舒胸片血清HPLC指纹图谱中可检出15个成分,其中血清本身具的成分4个,其余11个可能来自复方的原成分及其代谢产物。舒胸片中已知的6种主要成分(川芎嗪、羟基红花黄花素A、阿魏酸、三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1)均可从血清中检出,表明为入血成分。在血清HPLC指纹图谱与复方HPLC指纹图谱的基础上,建立了舒胸片的表观血清指纹图谱。结论:所建立的舒胸片提取液HPLC指纹图谱测定方...

Objective： To conduct serum pharmacochemistry study on extract in Shuxiong Tablets. Method： HPLC research on the serum fingerprints chromatograms of Shuxiong tablet extract was based on blood serum chemical pharmacy： After SD rats were administrated by gavage, samples were taken respectively after certain periods and measures were made on serum ehromatograms of both mixed extracts and each single extract, then analysis is made. An HPLC method was adopted with column Kromasil C18 （4.60 mm x200 ram, 5 trm ）. The mobile phase was consisted of...