期刊论文

中文

中国实验方剂学杂志

1005-9903

2016

22

23

1-5

10.13422/j.cnki.syfjx.2016230001

国家自然科学基金项目（81473581）；

本校为第一机构

目的:采用UPLC-MS研究黄芪-当归不同剂量配伍药液及小肠吸收液中5种化学成分(阿魏酸、毛蕊异黄酮苷、毛蕊异黄酮、芒柄花素、黄芪甲苷)的含量变化,为二者的临床配伍用药提供参考。方法:利用水提法制备黄芪-当归不同剂量配伍的药液,运用大鼠外翻肠囊法进行药物吸收试验制备药物的小肠吸收液,通过UPLC-MS测定药液和小肠吸收液中5种成分的含量,流动相0.1%甲酸水溶液-乙腈梯度洗脱,流速0.4 mL·min~(-1),进样量1 μL,毛细管电压4 kV。结果:黄芪-当归以10 ∶1和5∶1配伍时,5种成分的溶出量大多与理论值相近;当黄芪-当归以1∶1和1∶5配伍时,5种成分的溶出大多高于理论值。黄芪-当归10 ∶1和5∶1配伍时...

Objective： To study on the change of five chemical components（ ferulic acid,calycosin-7-O-β-D-glycoside,calycosin,formononetin and astragaloside Ⅳ） in solution and small intestine absorption liquid of Astragali Radix-Angelicae Sinensis Radix with different compatibility. Method： Decoction of Astragali Radix-Angelicae Sinensis Radix with different compatibility was prepared by water extraction method,the small intestinal absorption liquid was prepared by drug absorption experiment which was carried out with isolated everted intestinal meth...