期刊论文

Mechanism of Icariin in Inhibiting Osteoclast Differentiation Via ERα/RANK Signal Pathway

中文

中国实验方剂学杂志

1005-9903

2017

23

7

121-126

10.13422/j.cnki.syfjx.2017070121

国家自然科学基金面上项目（81273816） 中南大学研究生自主探索创新项目（2016zzts496）

本校为其他机构

目的:通过检测淫羊藿苷对破骨细胞诱导分化过程中雌激素受体α(estrogen receptor α,ERα),核转录因子-κB受体(receptor activator of nuclear factor-κB,RANK) mRNA表达的影响,探讨淫羊藿苷抑制破骨细胞分化作用机制。方法: 50 μg·L ~(- 1)核因子κB受体活化因子配体(receptor activator of nuclear factor-κB ligand,RANKL)体外诱导RAW264.7细胞分化成破骨细胞,利用抗酒石酸酸性磷酸酶(TRAP)染色和骨吸收陷窝对破骨细胞进行鉴定。用不同浓度的淫羊藿苷(1 × 10 ~(- 5),1 × 10 - 6,1 × 10 ~(- 7) mol·L ~(- 1))分别处理5 d和9 d后,进行TRAP染色和骨吸收陷窝分析,处理6 d后,利用逆转录...

Objective: To explore the mechanism of icariin in inhibiting the osteoclast differentiation via detecting the mRNA expression levels of estrogen receptor α (ERα) and receptor activator of NF-κB (RANK) in osteoclast induced from RAW264.7. Method: RAW264.7 cells were induced into osteoblasts with receptor activator of nuclear factor κB ligand (RANKL) (50 μg·L ~(- 1)),and were identified by tartrate resistant acid phosphatase (TRAP) staining and bone resorption pit formation. After RAW264.7 cells were treated with different concentrations (1...