期刊论文

Effect of Ginsenoside Rg_5 on Invasion and Migration of Gastric Cancer BGC-823 Cells via miR-125b/STARD13/NEU1 Signaling Pathway

中文

中国实验方剂学杂志

1005-9903

2018

24

22

138-142

10.13422/j.cnki.syfjx.20182026

湖南省教育厅课题项目（16C1242）；

本校为第一机构

目的:研究人参皂苷Rg_5对胃癌BGC-823细胞侵袭迁移的影响,并通过微小RNA-125b(microRNA-125b,miR-125b)/类固醇敏感性调节蛋白相关脂类转运体结构域13(StAR-related lipid transfer domain protein 13,STARD13)/唾液酸酶1(neuraminidase 1,NEU1)信号通路探讨人参皂苷Rg_5抑制胃癌侵袭迁移的作用机制。方法:人参皂苷Rg_5(12.5,25,50 μmol·L~(-1))处理BGC-823细胞后,采用transwell小室实验检测人参皂苷Rg_5对BGC-823细胞侵袭、迁移能力的影响,实时荧光定量PCR(Real-time PCR)检测miR-125b,STARD13,NEU1 mRNA的表达,蛋白免疫印迹法(Western blot)检测STARD13,NEU1蛋白的表达。结果:人参皂苷Rg_5(...

Objective:To study the effect of ginsenoside Rg_5(12.5,25,50 μmol·L~(-1)) on the invasion and migration and the expressions of microRNA-125b(miR-125b),StAR-related lipid transfer domain protein 13(STARD13) and neuraminidase 1 (NEU1)in gastric cancer BGC-823 cell, in order to investigate the possible mechanism of ginsenoside Rg_5 in inhibiting the invasion and migration of gastric cancer. Method: Transwell was used to detect the invasion and migration of gastric cancer BGC-823 cell, Real-time PCR was used to detect the mRNA expressions of miR-...