期刊论文

中文

中国实验方剂学杂志

1005-9903

2018

24

3

120-124

10.13422/j.cnki.syfjx.2018030120

湖南省自然科学基金项目（2016JJ4057） 湖南省中医药科研计划项目（201624）

本校为其他机构

目的:通过体内实验研究健脾消癌方对小鼠大肠癌皮下移植瘤细胞凋亡及相关蛋白表达的影响,探讨其抗大肠癌复发转移的作用机制。方法:通过皮下注射结肠癌细胞CT-26构建大肠癌皮下移植瘤小鼠模型,随机分为模型组,健脾消癌方低(15.75 g·kg~(-1)),中(31.5 g·kg~(-1)),高(63 g·kg~(-1))剂量组及5-氟尿嘧啶(5-Fu)组,分别给药14 d后,处死小鼠,剪取瘤体、称取瘤质量后剪碎瘤组织,采用流式细胞术检测各组细胞凋亡情况,并采用蛋白免疫印迹法(Western blot)检测细胞凋亡相关蛋白表达情况。结果:与模型组比较,健脾消癌方(15.75,31.5,63 g·kg~(-1))组瘤质量均减轻(P

Objective:To study the effect of Jianpi Xiaoai recipe in vivo on apoptosis of subcutaneous tumor cells and expression of related proteins in mice with colorectal carcinoma, and investigate its mechanisms of anti metastasis and recurrence of colorectal cancer. Method: By subcutaneous injection of CT-26 colon cancer cells to construct models of subcutaneous colorectal cancer, and then these mice were randomly divided into five groups: model group, Jianpi Xiaoai low(15.75 g·kg~(-1)), medium(31.5 g·kg~(-1)), high (63 g·kg~(-1)) dose groups, and ...