期刊论文

中文

中国实验方剂学杂志

1005-9903

2018

24

6

172-176

10.13422/j.cnki.syfjx.20180687

81373602:国家自然科学基金 2017JJ3186:湖南省自然科学基金青年项目 201604:湖南省中医药研究院重点项目 201504:湖南省中医药研究院重点项目

本校为第一机构

目的:观察重楼皂苷Ⅰ(polyphyllin Ⅰ,PPⅠ)对结肠癌HCT116细胞凋亡及凋亡相关因子Bcl-2相关X蛋白(Bcl-2 associated X protein,Bax),B细胞淋巴瘤-2(B cell lymphoma-2,Bcl-2),半胱天冬酶-3(Caspase-3)蛋白表达的影响,探讨重楼皂苷Ⅰ抑制结直肠癌转移的可能作用机制。方法:Cell counting kit-8(CCK-8)法检测12,24,48 h的细胞生长抑制作用,流式细胞术检测不同浓度的重楼皂苷Ⅰ对HCT116细胞凋亡的影响,蛋白免疫印迹法(Western blot)检测凋亡相关因子Bax,Bcl-2,Caspase-3蛋白的表达。结果:重楼皂苷Ⅰ可抑制对HCT116细胞生长,呈一定的浓度、时间依赖性;可促进HCT116细胞凋亡,呈一定的浓度依赖性。与...

Objective:To observe the effect of polyphyllin Ⅰ on the protein expression of Caspase-3 in the apoptosis of colon cancer HCT116 cells and apoptosis-related protein Bcl-2 associated X protein (Bax) and B cell lymphoma-2 (Bcl-2), in order to investigate the inhibitory effect of polyphyllin Ⅰ on the metastasis of colorectal cancer. Method: Cell counting kit-8(CCK-8) experiment was performed for the detection of the cell growth inhibition effect; flow cytometry was used to detect the apoptosis rate of HCT116 cells after the treatment with polyphy...