期刊论文

Mechanism of Jianpi Xiaoai Prescription in Inhibiting Colon Cancer Proliferation

中文

中国实验方剂学杂志

1005-9903

2018

24

6

151-155

10.13422/j.cnki.syfjx.20180683

81373602:国家自然科学基金 2017JJ3186:湖南省自然科学基金青年项目 201604:湖南省中医药研究院重点项目 201504:湖南省中医药研究院重点项目

本校为第一机构

目的:观察健脾消癌方对结肠癌HCT116细胞中微小RNA-21(microRNA-21,miR-21),第10号染色体丢失的磷酸酶基因(phasphatase and tensin homo-log deleted in chromosome 10,PTEN)表达的影响,探讨健脾消癌方抑制结肠癌转移的可能作用机制。方法:10%,15%,20%健脾消癌方含药血清处理HCT116细胞后,实时荧光定量PCR(Real-time PCR)检测miR-21,PTEN mRNA的表达水平,蛋白免疫印迹法(Western blot)检测PTEN蛋白的表达水平。结果:与空白组比较,健脾消癌方低剂量组miR-21 mRNA相对表达量降低,PTEN mRNA相对表达量升高,但差异无统计学意义;健脾消癌方中剂量组miR-21 mRNA相对表达明显降低,PTEN mRNA相对表达明...

Objective:To observe the effect of Jianpi Xiaoai prescription on the expressions of microRNA-21 (miR-21), phasphatase and tensin homo-log deleted in chromosome 10 (PTEN) in colon cancer HCT116 cells, in order to investigate the possible mechanism of Jianpi Xiaoai prescription on the metastasis of colon cancer. Method: After the treatment with drug serum (10%, 15% and 20%), Real-time PCR was used to detect the mRNA expressions of miR-21, PTEN, and the protein expression of PTEN was detected by Western blot. Result: Compared with control group, the relative mRNA expression of miR-21 was decrease...