期刊论文

Rapid Identification of Alkaloids in Evodia rutaecarpa by HPLC-Q-TOF-MS/MS

中文

中国实验方剂学杂志

1005-9903

2019

25

19

102-108

10.13422/j.cnki.syfjx.20190915

2013年中医药部门公共卫生专项(财社[2013]135号) 国家中药标准化项目(ZYBZH-Y-HUN-21).

本校为第一机构

目的:应用高效液相色谱-四级杆-飞行时间串联质谱法(HPLC-Q-TOF-MS/MS)快速获取吴茱萸中生物碱类组分信息。方法:采用Inter Sustain-C_(18)色谱柱(4.6 mm×250 mm,5 μm),以0.2%甲酸水-乙腈为流动相,梯度洗脱,柱温25 ℃,流速1.0 mL·min~(-1),进样量5 μL。检测波长245 nm,采用正、负离子2种模式对色谱流出物进行检测与分析。结果:通过分子离子峰和二级质谱特征碎片离子,结合对照品的质谱信息和相关文献报道,对40余个主要峰进行成分分析,最终从吴茱萸甲醇提取物中鉴定了21种生物碱,包括10种吲哚生物碱,10种喹诺酮生物碱,1种麻黄碱,基本明确了吴茱萸中生物碱的主要类型成分。并且研究发现这几种生...

Objective:To obtain the information of alkaloids in Evodia rutaecarpa by HPLC-Q-TOF-MS/MS.Method:Inter Sustain-C_(18) column (4.6 mm×250 mm,5 μm) was used with 0.2% formic acid water-acetonitrile as the mobile phase for gradient elution.The column temperature was 25 °C,the volume flow rate was 1.0 mL·min~(-1),and the sample volume is 5 μL.The detection wavelength was 245 nm,and the chromatographic effluent was detected and analyzed by using both positive and negative ions.Result:According to molecular ion peaks and secondary mass spectrome...