期刊论文

Effect of Drug-containing Serum of Clerodendrum bungei on Liver Cancer Cells Through PI3K/Akt Signaling Pathway

中文

中国实验方剂学杂志

1005-9903

2019

25

22

28-33

10.13422/j.cnki.syfjx.20191222

国家中医药管理重大项目(国中医药人教函[2012]228号) 湖南中医药大学校级科研项目(2017XJJJ21).

本校为第一机构

目的:从磷脂酰肌醇3-激酶（PI3K）/丝氨酸苏氨酸蛋白激酶（Akt）信号通路角度探讨臭牡丹含药血清对肝癌MHCC97-H细胞的影响及可能的机制。方法:臭牡丹15%含药血清作用于MHCC97-H细胞,细胞增殖/毒性法（CCK-8）观察臭牡丹含药血清对细胞增殖的影响,以筛选最佳作用时间和浓度; Annexin V-FITC/碘化丙啶（PI）双染法检测细胞凋亡;蛋白免疫印迹法（Western blot）检测臭牡丹含药血清作用于细胞72 h后对PI3K/Akt信号通路中关键蛋白中第10号染色体缺失的磷酸酶及张力蛋白同源的基因（PTEN）,磷酸化Akt（p-Akt）和磷脂酰肌醇3-激酶（PI3K）蛋白表达的影响;实时荧光定量聚合酶链式反应（Real-time PCR）...

Objective: To investigate the effect of Clerodendrum bungei-containing serum on liver cancer MHCC97-H cells and its possible mechanism from the perspective of phosphatidylinositol 3-kinase(PI3K)/protein kinase (Akt) signaling pathway.Method: The medicinal serum of 15% C. bungei was used to treat MHCC97-H cells. The effect of serum containing C. bungei on cell proliferation was observed by cell counting kit-8(CCK-8) method, in order to select the best time and concentration. The apoptosis was detected by Annexin V-FITC/PI double staining method....