期刊论文

Effect of Zhizhuwan on Intestinal Conduction and PLC-γ_1/PLC-γ_2 Signaling Pathway of Slow Transit Constipation Model Mouse with Spleen Deficiency Syndrome

中文

中国实验方剂学杂志

1005-9903

2019

25

10

8-14

10.13422/j.cnki.syfjx.20191039

国家自然科学基金青年基金项目(81603597).

本校为第一机构

目的:探讨枳术丸对脾虚证慢传输型便秘(STC)小鼠肠道传导功能的干预作用,及对小鼠结肠组织磷脂酶C-γ_1(PLC-γ_1)/磷脂酶C-γ_2(PLC-γ_2)信号通路表达的影响。方法:将无特定病原体(SPF)健康小鼠随机分为正常组与造模组。采用番泻叶灌胃造成脾虚状态,然后控制饮食饮水的方法造成脾虚便秘的小鼠模型,造模完成后将模型组小鼠随机分为模型组、枳术丸组、莫沙必利组,枳术丸组按9.0 g·kg~(-1)·d~(-1)给药,莫沙必利组按2.5 mg·kg~(-1)·d~(-1)给药,模型组和正常组给予等剂量的蒸馏水,连续给药7 d,治疗完成后用印度墨水在结肠中推进的长度计算小鼠肠道推进率;用D-木糖试剂盒测定小鼠血清D-木糖含量...

Objective: To explore the effect of Zhizhuwan on intestinal conduction and expressions of Phospholipase C-γ_1 (PLC-γ_1)/Phospholipase C-γ_2 (PLC-γ_2) signaling pathway of slow transit constipation (STC) with spleen deficiency syndrome. Method: Special pathogen free (SPF) healthy mice were randomly divided into normal group and model making group. Folium Sennae gavage was used to induce the spleen deficiency status, and then diet and drinking water were controlled to establish the mice model of spleen deficiency constipation. After the model...