期刊论文

Effect of Liuwei Dihuangtang on Mitochondrial Apoptotic Pathway in Rats with Chronic Renal Failure

中文

中国实验方剂学杂志

1005-9903

2020

26

22

26-33

10.13422/j.cnki.syfjx.20201875

国家自然科学基金青年基金项目(81503442) 研究生创新课题项目(2019CX13)。

本校为第一机构

目的:观察六味地黄汤对慢性肾衰竭大鼠线粒体凋亡途径的影响。方法:将45只雄性SD大鼠随机分为正常组(10只)、假手术组(10只)及手术组(25只),手术组进行5/6肾切除造模,造模成功后再随机分为模型组及治疗组。治疗组予以6.75 g·kg~(-1)六味地黄汤灌胃,其余3组予以等体积蒸馏水灌胃,各组均给药8周。检测各组大鼠血肌酐、血尿素氮及尿蛋白定量水平;苏木素-伊红(HE),马松(Masson)染色观察肾组织形态学变化;电镜观察肾小管上皮细胞中线粒体超微结构变化;原位末端标记法(TUNEL)检测细胞凋亡;蛋白免疫印迹法(Western blot)及实时荧光定量聚合酶链式反应(Real-time PCR)分别检测肾组织B淋巴细胞瘤-2(Bcl-...

Objective:To observe the effect of Liuwei Dihuangtang on the mitochondrial apoptotic pathway in rats with chronic renal failure.Method:The 45 male SD rats were randomly divided into normal group(n=10),sham group(n=10)and operation group(n=25).The operation group received 5/6 nephrectomy,and the rats in operation group were randomly divided into the model group and the treatment group after successful modeling.The treatment group received Liuwei Dihuangtang by gavage administration,while the rest of the three groups received equal volume of distilled water by gavage administration,with a treatm...