期刊论文

Efficacy of Zhizhuwan Decoction and Its Ingredient-contained Serums on Proliferation and Apoptosis of Rat Colon Interstitial Cells of Cajal

中文

中国实验方剂学杂志

1005-9903

2020

26

19

120-126

10.13422/j.cnki.syfjx.20201709

国家自然科学基金青年基金项目(81603597) 湖南省教育厅科学研究项目(17A161) 湖南省中医药科研计划项目(201954)。

本校为第一机构

目:探讨枳术丸水煎液及其拆方对大鼠结肠Cajal间质细胞(ICC)增殖和凋亡的影响及其分子机制,并从细胞分子层面分析中医攻补兼施法治疗慢传输型便秘可能的机制。方法:将40只大鼠随机分成白术组、枳实组、枳术丸组、空白血清组,每组10只。白术组灌以17.7g·kg~(-1)·d~(-1)白术水煎液,枳实组灌以8.9g·kg~(-1)·d~(-1)枳实水煎液,枳术丸组灌以26.4g·kg~(-1)·d~(-1)枳术丸水煎液,空白血清组灌以无菌蒸馏水3mL,每日1次,连续7d,取血制备含药血清和空白血清。将4组含药血清分别配成5%,10%,15%,20% 4个体积分数,干预24h,观察ICC的数量、形态,用细胞增殖毒性检测试剂盒(CCK-8)检测各组细胞增殖情况,测定...

Objective:To explore the efficacy and molecular mechanism of Zhizhuwan decoction and its ingredient-contained serums on the proliferation and apoptosis of rat colon interstitial cells of cajal(ICC),and make a molecule-level analysis of the possible mechanism of traditional Chinese medicine (TCM) purgation-tonifying therapy in treating slow transit constipation(STC).Method:A total of 40 rats were divided into Atractylodis Macrocephalae Rhizoma(AMR)group,Aurantii Fructus Immaturus(AFI)group,Zhizhuwan group and blank serum group on random basis,wi...