期刊论文

Effect of Yuehua Capsule on Autophagy of Macrophage Infected with Multi-drug Resistant Mycobacterium Tuberculosis

中文

中国实验方剂学杂志

1005-9903

2020

26

2

72-79

10.13422/j.cnki.syfjx.20200241

国家自然科学基金项目(81470192) 湖南省教育厅科学研究重点项目(13A065)。

本校为其他机构

目的:以月华胶囊含药血清对耐多药结核分支杆菌感染小鼠单核巨噬细胞(RAW264.7)进行干预,探讨其对耐多药结核分支杆菌感染巨噬细胞自噬的作用及其机制。方法:低温冷冻干燥法制备月华胶囊,大鼠按3.02 g·kg~(-1)灌胃给药7 d,制备月华胶囊含药血清。体外培养RAW264.7,耐多药结核分子杆菌。以细胞增殖毒性检测试剂盒(CCK-8)检测含药血清对RAW264.7的增殖能力并选定其有效浓度。细胞分为模型组(10%胎牛血清);月华胶囊含药血清组(10%月华胶囊);自噬抑制剂3-甲基腺嘌呤(3-MA)+月华胶囊含药血清组(5 mg·L~(-1)的3-MA+10%月华胶囊含药血清);雷帕霉素(Rap)组(200 mg·L~(-1)的Rap+10%胎牛血清);正常组(1...

Objective: This study aims to explore the effect and mechanism of Yuehua capsule serum for autophagy of macrophages infected with multi-drug resistant mycobacterium tuberculosis.Method: The rats were undertaken intragastric gavage with Yuehua capsule by 3.02 g·kg~(-1)once a day which was produced through low temperature condensation drying method. After 7 days, blood of abdominal aorta of rats was collected to prepare Yuehua capsule serum. RAW264.7 andmultidrug resistant tuberculosis were cultured in vitro.According to cell counting kit-8(CCK-...