期刊论文

Quality Control of Dynamic Volatile Components in Lonicerae Japonicae Flos and Lonicerae Flos

中文

中国实验方剂学杂志

1005-9903

2020

26

22

148-155

10.13422/j.cnki.syfjx.20201047

国家自然科学基金项目(81703824) 中药成药性与制剂制备湖南省重点实验室开放基金项目(201705) 湖南中医药大学研究生创新项目(2018CX51) 湖南中医药大学大学生创新训练项目。

本校为第一机构

目的:运用分子连接性指数法与总量统计矩法控制中药质量,阐明金银花、山银花挥发性成分的稳定性与一致性。方法:提取不同产地金银花、山银花挥发油进行GC-MS测定,采用电子轰击离子源,离子源温度230 ℃,检测范围m/z 35~ 650;结合美国国家标准与技术局(NIST)05和ChemicalBook数据库定性分析挥发性成分,采用峰面积归一化法进行定量分析并计算总量统计矩参数和挥发性成分的零阶、一阶、二阶、三阶分子连接性指数。结果:10批金银花、山银花各指纹图谱峰数目(RSD分别为28.5%,33.4%),总量零阶矩(RSD分别为55.5%,128.9%)和总量二阶矩(RSD分别为15.3%,21.5%)不稳定,表明挥发性成分种类与含量波动剧烈;但总...

Objective:The molecular connectivity index method and total statistical moment method were used to control the quality of traditional Chinese medicine(TCM),and the stability and consistency of volatile components of Lonicerae Japonicae Flos and Lonicerae Flos were clarified.Method:Volatile oils in Lonicerae Japonicae Flos and Lonicerae Flos from different producing areas was extracted for GC-MS determination with electron bombardment ion source,ion source temperature of 230 ℃,and detection range of m/z 35-650.Then National Institute of Standar...