期刊论文

Mechanism of Modified Si Junzitang on Neuronal Anti-anoikis of Cells in Rats with Cerebral Ischemia Through Fibulin-5 and p-Akt

Li, H.

中文

中国实验方剂学杂志

1005-9903

2021

27

1

112-120

10.13422/j.cnki.syfjx.20210139

81473567:国家自然科学基金 2019JJ40219:湖南省自然科学基金 202008:湖南省中医药科研重点项目 422:湖南省大学生研究性学习和创新性实验计划

本校为第一机构

目的:探讨加味四君子汤对大鼠脑缺血再灌注(I/R)损伤后缺血侧海马区脑组织纤维蛋白-5(Fibulin-5),磷酸化蛋白激酶B(p-Akt)的表达及对神经细胞失巢凋亡的影响。方法:将60只SPF级雄性SD大鼠随机分为假手术组、模型组、依达拉奉组(3.2 mg·kg~(-1)),加味四君子汤高、中、低剂量组(19.08,9.54,4.77 g·kg~(-1))。采用线栓法制备大鼠大脑中动脉栓塞(MCAO)模型,7 d后处死大鼠,处死前进行神经功能缺损评分,采用苏木素-伊红(HE)染色进行组织病理学观察,原位末端标记法(TUNEL)染色检测神经细胞凋亡指数,免疫组化法和蛋白免疫印迹法(Western blot)检测缺血侧海马区脑组织Fibulin-5及p-Akt蛋白表达情况。结...

Objective: To investigate the effect of modified Si Junzitang on the expression of fibrous protein-5 (Fibulin-5), phosphorylated protein kinase B (p-Akt) in hippocampus of rats after cerebral ischemiareperfusion (I/R) injury and the anoikis of nerve cells. Method: The 60 male SD rats of SPF grade were randomly divided into sham operation group, model group, Edaravone group (3.2 mg·• kg-1) and modified Si Junzitang high, medium and low-dose groups (19.08, 9.54, 4.77 g·• kg-1). The middle cerebral artery occlusion (MCAO) model was established...