期刊论文

Effect of Shugan Jianpi Jiedu Prescription Medicated Serum on Proliferation,Migration and Invasion of Triple-negative Breast Cancer MDA-MB-231 Cells Based on PI3K/Akt/mTOR Signaling Pathway

Bai, Z.-P.

中文

中国实验方剂学杂志

1005-9903

2021

27

15

22-28

10.13422/j.cnki.syfjx.20211592

湖南省教育厅科学研究项目（18C0362）； 湖南省卫生健康委科研计划项目（20200230）； 长沙市科技计划项目（kq2004048）；

本校为第一且通讯机构

目的:通过体外细胞实验研究疏肝健脾解毒方对三阴乳腺癌治疗的有效性和可能机制。方法:实验设置正常血清组、吡柔比星组和低、中、高剂量疏肝健脾解毒方含药血清组。将20只SD大鼠随机分成4组,按照体表面积换算分别予以16.8,8.2,4.05 g·kg~(-1)的疏肝健脾解毒方药液和等体积的生理盐水灌胃制备血清。取血清配置各组药液后,分别处理MDAMB- 231细胞。利用细胞增殖与活性检测-8(CCK-8),细胞划痕法和细胞侵袭实验(transwell)法检测其增殖、迁移和侵袭能力,通过蛋白免疫印迹法(Western blot)检测MDA-MB-231细胞中磷脂酰肌醇3-激酶(PI3K),蛋白激酶B(Akt)和雷帕霉素靶蛋白(mTOR)蛋白表达水平。结果:与正...

Objective： To study the efficacy and mechanism of Shugan Jianpi Jiedu prescription（SJJ） in the treatment of triple-negative breast cancer through in vitro cell experiments. Method： The following groups were set up in this study：a normal serum group，a pirarubicin group，and low-，medium-，and high-dose SJJ-medicated serum groups. Twenty SD rats were randomly divided into four groups and administered with SJJ solution（16.8，8.2，4.05 g·kg-1 ）and normal saline（equal volume）according to the body surface area to prepare serum. MDA-MB-231 ...